Winthrop is bordered by 3 major roads: Leach Highway to the north, Murdoch Drive to the east, North Lake Road to the west and Somerville Boulevard to the south, which connects North Lake Road to Murdoch Drive.
The main local neighbourhood distributors are: Winthrop Drive, dividing the east and west of Winthrop, and Jackson Avenue, which passes both Winthrop Primary School and the Winthrop Village Shopping Centre.
There are numerous bus routes serving Winthrop. These are:
- 503 (Bull Creek – Murdoch)
- 504 (Bull Creek – Murdoch)
- 511 (Murdoch – Fremantle)
- 940 (Hamilton Hill – Wellington Bus Station)
The closest train stations are Bull Creek and Murdoch, both situated 1.2 km east of Winthrop. Fremantle Train Station is located approximately 9 km to the west.